Extended Data Fig. 3: Gene expression profile induced by CAR-derived tonic signals.
a, Principal component analysis (PCA), b, number of Differentially Expressed Genes (DEGs) significantly up- and down-regulated by each CAR versus Mock and c, volcano plots showing the differentially expressed genes between CAR and Mock cells from three different donors 6 days after CAR transduction. d, Heatmap of pathway analysis from each CAR construct compared to Mock. e, Heatmaps of the pathway analysis (Reactome, Consensus, Hallmark and GO) regulated by each single CAR construct compared to BB. f, Venn diagrams indicate the number of DEGs changed for each construct or shared among them. Every CAR is compared to Mock. g, Genes exclusively regulated by a single CAR construct. (d, e) Two-sample t-test, * indicates statistical significance, p < 0.05. Exact p values are provided in the source data file. n = 3 independent donors.
