Fig. 3: Exclusive and shared gene expression profiles on antigen encounter.
a, PCA. b,c, Pathway analysis of primary T cells expressing BB CAR versus Mock (b) and number of DEGs significantly up- and downregulated (c) comparing cells expressing every CAR stimulated versus BB 24 h after stimulation with CD19+ Nalm6 cells. d, Heatmap of the pathway analysis performed for each CAR construct compared with BB after 24 h of stimulation. e, Venn diagrams indicating the number of DEGs changed for each construct or shared among them. Every CAR is compared with the BB CAR 24 h after stimulation. f,g, Number of DEGs significantly up- or downregulated (f) and heatmap of the pathway analysis (g) performed for BBζ versus BBε and BBγ versus BBδ. Stimulation conditions are indicated. Three independent donors were analyzed in all panels. For d and g, a two-sample Student’s t-test was used. *Indicates statistical significance; P < 0.05. Exact P values are provided in Source data.
