Extended Data Fig. 1: CARs containing the CD3δ/ε/γ ICD are functional.
a, Scheme depicting the ICD of all CD3 chains used in this study and the localization of the cysteines driving CAR dimerization. b, Specific killing of CD19+ Nalm6 cells by primary human T cells lentivirally transduced with the indicated CAR after 24 h of co-incubation (1:1 ratio). Each dot represents an independent donor (n = 4). c, Schematic representation of the in vivo model. Data are represented as mean ± s.d. and analyzed by paired one-way ANOVA and Dunnett’s multiple comparisons test. ITAM, Immunoreceptor Tyrosine-based Activation Motif; BRS, Basic Rich Stretch; PRS, Proline Rich Sequence; RK, Receptor Kinase; ERR, endoplasmic reticulum retention.
