Extended Data Fig. 5: BRWD1 modulates cohesin binding to mediate static to dynamic cohesin conversion.
From: BRWD1 orchestrates small pre-B cell chromatin topology by converting static to dynamic cohesin
a, Bar diagram showing expression (RNA-seq; n = 4) of indicated cohesin component genes in B cell progenitor subsets. Data are represented as mean ± SD. b, c, Total and coincident genomic peaks for RAD21 and SMC3 in WT (b) and Brwd1-/- (c) small pre-B cells. The total number of peaks for each population is shown in parentheses with the number in each Venn region indicated. d, e, Total and overlapping coincident genomic peaks for RAD21, SMC3 and NIPBL in WT (d) and Brwd1-/- (e) small pre-B cells. f-g, Total and overlapping coincident genomic peaks for RAD21, SMC3 and WAPL in WT (f) and Brwd1-/- (g) small pre-B cells. h-i, Total and overlapping coincident genomic peaks for RAD21 + SMC3 complex to NIPBL and WAPL in WT (h) and Brwd1-/- (i) small pre-B cells. j, HiC interaction matrix for the indicated genomic region displaying BRWD1 binding near or at TAD anchor sites with CTCF binding (black arrowhead). Red and blue arrows indicate CTCF direction. k, HiC interaction matrix for indicated genomic region along with ChIP-seq profile of BRWD1 binding (WT), CTCF, RAD21, SMC3, NIPBL and WAPL binding in WT and Brwd1-/- small pre-B cells for the indicated region shown by a black bar. Pink shaded boxes indicate regions with 4 F (RAD21 + SMC3 + NIPBL + WAPL; dynamic cohesin) binding in both WT and Brwd1-/- small pre-B cells near or at CTCF sites. Yellow boxes denote regions with 4 F sites in WT small pre-B cells but 2 F (RAD21 + SMC3; static cohesin) sites in Brwd1-/- small pre-B cells near/at CTCF binding sites. Green boxes denote 4 F (RAD21 + SMC3 + NIPBL + WAPL, without CTCF; dynamic cohesin) sites in WT small pre-B cells but without any detectable cohesin binding in Brwd1-/- small pre-B cells.
