Extended Data Fig. 6: BRWD1 mediates loop extrusion at Igk locus by recruiting dynamic cohesin. | Nature Immunology

Extended Data Fig. 6: BRWD1 mediates loop extrusion at Igk locus by recruiting dynamic cohesin.

From: BRWD1 orchestrates small pre-B cell chromatin topology by converting static to dynamic cohesin

Extended Data Fig. 6: BRWD1 mediates loop extrusion at Igk locus by recruiting dynamic cohesin.The alternative text for this image may have been generated using AI.

a, HiC interaction matrix for the Igk locus demonstrating a stripe across the entire WT Igk locus (~3.2 Mb, half arrow) that is anchored at the recombination center. b, ChIP-seq binding profile of BRWD1 (in WT), CTCF, RAD21, SMC3, NIPBL and WAPL along with histone marks H3K4me1 and H3K27Ac in WT and Brwd1-/- small pre-B cells across the Igk locus. Shaded boxes show the presence of dynamic cohesin complex sites without CTCF binding sites in WT small pre-B cells but absent in Brwd1-/- small pre-B cells. c, Point of view arc plots displaying normalized genomic interactions at indicated Vk gene segments in WT (blue) and Brwd1-/- small pre-B cells. The location of the recombination center is shown. The height of the arcs indicates the intensity of interactions.

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