Extended Data Fig. 7: Cohesin subunit chromatin binding distributions in Brwd1-/- and ATP-depleted WT small pre-B cells.
From: BRWD1 orchestrates small pre-B cell chromatin topology by converting static to dynamic cohesin
a, Flow cytometry demonstrating the percentage of live cells in WT small pre-B cells untreated (left) and treated with oligomycin A (126 nM) for 2 hours (right). b, Bar plot displaying ATP depletion (%) in WT small pre-B cells after 2 hours of treatment with oligomycin A (126 nM). c-f, Total and coincident binding peaks for RAD21 (c), SMC3 (d), NIPBL (e) and WAPL (f) in WT, Brwd1-/- and ATP-depleted WT small pre-B cells. g, Total and coincident binding peaks for RAD21 and SMC3 in ATP-depleted WT small pre-B cells. h, Total and coincident binding peaks for RAD21 + SMC3 in WT, Brwd1-/- and ATP-depleted WT small pre-B cells. i, Total and coincident binding peaks for RAD21 + SMC3 with NIPBL and WAPL in ATP-depleted small pre-B cells.
