Extended Data Fig. 4: Rip2−/− mice do not display lymphopenia, decreased cytokine responsiveness or increased susceptibility to T. gondii infection.
From: Microbial ligand-independent regulation of lymphopoiesis by NOD1
a-b, Total cell number in spleen, thymus and BM (a) and number of LT-HSC, ST-HSC, CLP and CMP in BM (b) of age-matched WT and Rip2−/− animals (n = 6-9 and 4–6 in a and b, respectively). Each symbol represents values obtained for an individual mouse and bars indicate the mean value ± s.e.m. for each genotype pooled from two independent experiments performed. c, Proliferative response of FACS-purified LT-HSC (left) and CLP (right) stimulated with indicated hematopoietic cytokines was measured. Bars indicate mean ± S.D. values for at least duplicate cultures for one representative out of two performed. d, Survival kinetics of WT (n = 14), NOD1−/− (n = 4) or Rip2−/− (n = 10) mice infected i.p. with ME-49 cysts. Significance was determined by Log-rank (Mantel-Cox) test *P < 0.05, **P < 0.01.
