Extended Data Fig. 9: Compromised T cell functions in ΔCARD NOD1 mice.
From: Microbial ligand-independent regulation of lymphopoiesis by NOD1
a–c, CD4+ T lymphocytes from ΔCARD NOD1 mice fail to induce colitis in an adoptive transfer model. (a) Kinetics of body weight (mean ± s.e.m.) loss after transfer of CD4+ T cells from WT or ΔCARD NOD1 mice into RAG−/− recipients (n = 5/group). (b) Histological score and representative photomicrographs of hematoxylin and eosin-stained sections of ileum from recipients on day 69 post-transfer (bar = 400 μm). (c) Number of donor T cells recovered at the same time point is shown as FACS contour plots for splenocytes from one representative mouse. Each symbol represents a value obtained for an individual mouse from a single experiment and bars depicts the mean ± s.e.m. values for a group. d–f, ΔCARD NOD1 animals display increased susceptibility to tumor challenge. (d) B16 tumors in WT or ΔCARD NOD1 animals and (e) representative photomicrographs of a hematoxylin and eosin-stained section of a tumor from each group (bar = 3 mm) on day 14 after injection. (f) Kinetics of tumor size in WT or ΔCARD NOD1 mice. Each symbol depicts a value obtained for an individual mouse (n = 8) pooled from two independent experiments performed. (g) Quantification of necrotic areas in tumors on day 14 post-injection (n = 5) from one of two experiments. Bars indicate the mean ± s.e.m. values. (b,c,f,g) Data were analyzed using a two-sided Mann-Whitney test. *P < 0.05, **P < 0.01.
