Extended Data Fig. 2: Role of IL-1β, TNFα, OSM in inducing MmSC inflammatory program. | Nature Immunology

Extended Data Fig. 2: Role of IL-1β, TNFα, OSM in inducing MmSC inflammatory program.

From: A discrete ‘early-responder’ stromal-cell subtype orchestrates immunocyte recruitment to injured tissue

Extended Data Fig. 2

a, Population-level RNA-seq of MmSCs isolated at 2 (n = 2), 4 (n = 1) or 8 (n = 2) hrs after ip co-injection of IL-1β, TNFα, OSM and IL-17A vs after PBS injection (n = 2). Expression levels across time points of the top 20 transcripts from the 100-gene inflammatory signature most differentially expressed. Y-axes plot values in arbitrary units. Each data point represents an individual mouse. b, Violin plots of Il1b, Tnf and Osm expression across cell populations in skeletal muscle on D0.5 after CTX-induced injury. scRNA-seq dataset as per16. Cell nomenclature as per original dataset. c, Gating strategy for the cytofluorimetric analysis of diverse immunocyte populations from hindlimb muscles. AU, arbitrary units; APC, antigen-presenting cells; FAPs, fibro/adipogenic progenitors; M1, inflammatory MFs; MuSC, muscle stem cells; PBS, phosphate-buffered saline; FC, fold change.

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