Extended Data Fig. 3: Functionality of CD4⁺ T Cells isolated from Scd1^+/- and Scd1^-/- mice.

(a) The percentage of CD44⁻CD62L⁺, CD44⁺CD62L⁻, and CD44⁺CD62L⁺ CD4⁺ T cells in the spleen of Scd1^+/- (n = 7) and Scd1^-/- mice (n = 5) at steady status. (b) CFSE-labeled splenic CD4⁺CD25⁻ conventional T cells from Scd1^+/- and Scd1^-/- mice were subjected to anti-CD3/CD28 stimulation in vitro for 3 days and cell proliferation was detected by assessing CFSE dilution. (c-f) Splenic CD4⁺CD62L⁺T cells from Scd1^-/- and Scd1^+/- mice were cultured in Th1, Th2, or Th17 induction medium for 3 days (n = 3 for Th1 and Th17 differentiation, n = 4 for Th2 differentiation). The percentages of IFNγ⁺, IL-4⁺, or IL-17⁺ CD4⁺T cells were determined by flow cytometry. Data are presented as Mean ± SEM. NS, not significant; P values were determined by unpaired two-tailed Student’s t-test.

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