Extended Data Fig. 4: Treg cells are comparable in CD4^creScd1^loxP and Scd1^loxP mice.

(a) mRNA levels of Scd1 in CD4⁺ T cells and hepatocytes isolated from CD4^creScd1^loxP mice and Scd1^loxP littermate control mice (n = 5 mice for each group). (b) The percentages of stearic acid (18:0) and oleic acid (18:1n9) among total fatty acids in in vitro expanded CD4⁺ T cells isolated from CD4^creScd1^loxP mice and Scd1^loxP mice (n = 3 for each group). (c) The CD4⁺ T cell reconstitution efficiency in Rag2^-/- mice receiving co-transfer of CD45.1⁺WT CD4⁺ T cells and CD45.2⁺CD4^creScd1^loxP CD4⁺ T cells (n = 4 mice per group). (d) The percentage of Treg cells among CD4⁺ T cells in the spleen, auxiliary lymph node and central nervous system of CD4^creScd1^loxP mice and Scd1^loxP mice on day 0 (steady, n = 3 mice per group), day 18 (peak, n = 5 for Scd1^loxP mice, n = 3 for CD4^creScd1^loxP mice), and day 24 (recovery, n = 5 for Scd1^loxP mice, n = 3 for CD4^creScd1^loxP mice) post EAE induction. Data are presented as Mean ± SEM. NS, not significant; P values were determined by unpaired two-tailed Student’s t-test.

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