Extended Data Fig. 5: The T cell reconstitution efficiency of WT and Scd1-/- bone marrow cells were comparable.
(a) Representative gating strategies in analyzing the thymocyte populations of the recipient mice as treated in Fig. 2a. The reconstitution efficiency of CD45.1+ and CD45.1+CD45.2+ cells within thymocytes at DN, DP, and SP stages were depicted. (b) The reconstitution efficiency of CD45.1+WT and CD45.1+CD45.2+Scd1-/- bone marrow cells transplantation (BMT) in different tissues of recipient mice as treated in Fig. 2a. (c) The percentages of donor CD4+ cells among CD45+ cells in the blood and different organs of recipient mice in Fig. 2a. (d) The thymic CD4−CD8− double negative (DN) subpopulation levels in Scd1+/- mice and Scd1-/- mice (n = 5 mice for each group). (e) The thymic DN, CD8SP, CD4SP, and DP levels in Scd1+/- mice and Scd1-/- mice (n = 6 mice for each group). Data are presented as Mean ± SEM. NS, not significant; P values were determined by unpaired two-tailed Student’s t-test.
