Extended Data Fig. 6: DN3 and DN4 cells of Scd1 deficient origin generated CD4+ T cells with intrinsic Treg propensities upon injection into the thymus of WT mice.
(a) Gating strategies (with the spleen as a representative sample) used to analyze the Treg cell proportion among donor-derived CD4+ T cells. (b-c) Thymocyte population from CD45.2+Scd1-/- mice and CD45.1+WT mice at the DN1, DN2, DN3, or DN4 stage were mixed at a 1:1 ratio and co-transferred into irradiated (3 Gy) CD45.1+CD45.2+ WT recipient mice via intrathymic injection (n = 5 mice for DN1, DN2 transfer; n = 6 mice for DN3, DN4 transfer). Four weeks post transfer, the recipient mice received WT and Scd1-/- DN thymocytes were euthanized to numerate the absolute number of CD4+ T cells (b) and CD4+Foxp3+ Treg cells (c) in various organs. Data are presented as Mean ± SEM. NS, not significant; P values were determined by paired (b, c) two-tailed Student’s t-test.
