Extended Data Fig. 8: The impact of Scd1 deficiency on the health status and thymic epithelial cells.

(a) Weight of body, spleen, and MLNs of K14^creScd1^loxP and Scd1^loxP mice (n = 5 mice for each group). (b-c) The whole image and H&E staining of various organs of K14^creScd1^loxP and Scd1^loxP mice. (d) Gating strategies used for identifying cortical thymic epithelial cells (cTEC) and medullar thymic epithelial cells (mTEC) of Scd1^+/- and Scd1^-/- mice, with subsequent calculation of their absolute numbers (n = 3 mice for each group). (e-f) cTEC and mTEC of Scd1^+/- and Scd1^-/- mice were isolated and subjected to mRNA sequencing analysis. The expression levels of critical regulators governing thymocyte migration, development, and selection in thymic epithelial cells were shown (e). The differentially expressed genes in cTEC or mTEC of Scd1^+/- and Scd1^-/- mice were shown in a volcano plot (f). (g) The percentage and absolute number of Helios⁻RORγt⁺Foxp3⁺ and Helios⁺ RORγt⁻Foxp3⁺ cells in the colon of K14^creScd^loxP (n = 5) and Scd1^loxP (n = 7) mice. (h-i) Average MALDI-TOF mass spectra of oleic acid in Fig. 4a. Data are presented as Mean ± SEM. NS, not significant; P values were determined by unpaired two-tailed Student’s t-test.

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