Extended Data Fig. 1: Properties of Treg cells of Scd1-/- mice.
(a) EAE was induced in Scd1-/- and Scd1+/- mice as described in Fig. 1a. The levels of Th17 and Treg cells in the central nervous system (CNS) were analyzed on day 17 post EAE induction (n = 7 mice for Scd1+/-+Iso, Scd1-/-+Iso, n = 5 mice for Scd1+/-+α-CD25, n = 6 for Scd1-/-+α-CD25). (b) The absolute number of CD4+Foxp3+ Treg cells and CD4+Foxp3− conventional T cells in various organs of Scd1-/- and Scd1+/- mice (n = 4 mice per group). (c) CD4+CD25+ T cells were isolated from the spleen of Scd1-/- mice and Scd1+/- littermate control mice, and co-cultured with activated CFSE-labeled CD4+ T cells (1 × 106) from wild type (WT) mice at indicated ratio. The proliferation of CD4+ T cells were determined by the dilution of CFSE. (d) The expression levels of CD25, CTLA-4, and GITR in CD4+Foxp3+ Treg cells were determined by flow cytometry. (e) Gating strategies (with the spleen as a representative example) for analyzing Helios expression in Treg cells. (f-g) The percentage and absolute number of Helios+ and Helios− Treg cells in the various tissues of Scd1-/- mice and Scd1+/- mice under steady status (n = 4 mice per group). Data are presented as Mean ± SEM. NS, not significant; P values were determined by unpaired two-tailed Student’s t-test.
