Extended Data Fig. 5: CD55-mediated extraction of CD97 NTF under shear stress is conserved on human MZ B cells.
From: Dynamic encounters with red blood cells trigger splenic marginal zone B cell retention and function
a, Structural components of the CD97-GFP fusion protein. NTF, N-terminal fragment; CTF, C-terminal fragment; GPS, GPCR proteolysis site; triangles indicate individual epidermal growth factor (EGF) domains; GFP, Green fluorescent protein. b,c, Splenocytes from CD97-GFP fusion protein expressing BM chimeras were cocultured for 45 minutes at 24 °C with Cd55+/+ RBCs, on a shaker or not. Representative histogram (left panel) and MFI (right panel) of surface CD97 expression (b) and GFP (c) on MZ B cells (n = 9 in each group). Data are pooled from two independent experiments. Each symbol represents one incubation and each donor contributes three incubations. Lines denote means, and line colors in histograms are for conditions of the types shown by color codes beneath the graphs. Statistical significance was tested by one-way ANOVA followed by Tukey’s multiple comparisons test. d, Dot plot illustrating marker gene expression for human specific B cell subtypes. Data are from GSE193869 (ref. 33). e, Representative flow cytometry profiles displaying gates of human naïve and MZ B cells. f, Representative histogram plot of surface CD55 on human peripheral RBCs.
