Extended Data Fig. 4: Type 1 Il1rl1 promoter deficiency abrogates ST2 expression by in vitro activated NKT cells and NK cells.
From: A type 1 immunity-restricted promoter of the IL−33 receptor gene directs antiviral T-cell responses
a-c, Thymic CD1d(α-GalCer-loaded)-Tetramer+ NKT cells were flow-cytometrically sorted from WT, Il1rl1 -/- or Il1rl1-ExAB-/- mice and stimulated in vitro with antibodies against CD3ε and CD28 in CTL/Th1 culture medium for 6 days. a, Representative FACS plots showing the purity of NKT cells after MACS pre-enrichment and after FACS sorting. b,c, Representative FACS plots (b) and quantification (c) of ST2 expression by NKT cells (WT: n = 5, Il1rl1-/-: n = 4, Il1rl1-ExAB-/-: n = 6). d-f, Splenic NKp46+ NK cells were flow-cytometrically sorted from CD4-, CD8- and B220-depleted splenocytes and stimulated with IL-12 + IL-33 for 48 h. d, Representative FACS plots showing the purity of NK cells after FACS sorting. e,f, Representative FACS plots (e) and quantification (f) of ST2 expression by NK cells (WT: n = 6, Il1rl1-/-: n = 3, Il1rl1-ExAB-/-: n = 6). Data are pooled from two independent experiments and are presented as mean ± SD with each dot representing one mouse (c,f). P was determined using one-way ANOVA with Tukey’s post hoc test (c,f).
