Extended Data Fig. 6: The type 1 Il1rl1 promoter drives expansion and activation of antiviral T cells.
From: A type 1 immunity-restricted promoter of the IL−33 receptor gene directs antiviral T-cell responses
a-i, WT, Il1rl1-/-, Il1rl1-ExAB-/- and Il1rl1-ExC-/- mice were infected with LCMV-WE and analyzed on d7 p.i. (WT: n = 9, Il1rl1-/-: n = 6, Il1rl1-ExAB-/-: n = 8, Il1rl1-ExC-/-: n = 7). a, Experimental outline. b,c, Representative FACS plots (b) and quantification (c) of liver CTLs. d-f, Representative FACS plots (d) and quantification of ST2 expression (e) and LCMV-Tetramer+ CTLs in livers (f). g-i, Representative FACS plots (g) and quantification (h,i) of effector molecule+ CTLs restimulated with LCMV GP33-41. j-m, Irradiated WT recipients (CD45.1+) were reconstituted with WT (CD45.1+ CD45.2+) and Il1rl1-/-, Il1rl1-ExAB-/- or Il1rl1-ExC-/- (all CD45.2+) bone marrow, infected with LCMV-WE and analyzed on d10 p.i. (WT+Il1rl1-/-: n = 6, WT+Il1rl1-ExAB-/- and WT+Il1rl1-ExC-/-: n = 7). j, Experimental outline. k, Counts of LCMV GP33-41-specific CTLs. l,m Frequencies and counts of SLECs (l) and MPECs (m). n-r, Smarta cells (CD90.1+) were cotransferred with Il1rl1-/- or Il1rl1-ExAB-/- Smarta cells (CD90.1+ CD90.2+) into WT mice (CD90.2+). Recipients were infected with LCMV-Cl13 and analyzed at d10 p.i. (Il1rl1-ExAB-/- Smarta: n = 6, Il1rl1-/- Smarta: n = 5). n, Experimental outline. o,p, Representative FACS plots showing CD4+ T-cell populations before transfer (o) and after infection (p). q,r, Frequencies (q) and counts (r) of Smarta cells in livers. s-v, Smarta, Il1rl1-/- Smarta or Il1rl1-ExAB-/- Smarta T cells (CD90.1+) were cotransferred into WT mice (CD90.2+). Recipients were infected with LCMV-Cl13 and analyzed at d8-9 p.i. s, Experimental outline. t,u, Representative FACS plots (t) and quantification of ST2 expression (u) (n = 6). v, Transferred cells were sorted and Il1rl1 first exon usage was quantified (Smarta: n = 4 with two samples <LOQ in Exon 1a and 1b reactions; Il1rl1-ExAB-/-: n = 4 with three samples <LOQ in Exon AB reaction; CTL, Th2, and NIH3T3 controls: n = 1). Data represent one (j-v), two (a-f) or three (g-i) independent experiments and are presented as mean ± SD with each dot representing one mouse. P was determined using two-tailed t-tests (u), one-way ANOVA with Tukey’s post hoc test (c,e,h,i), two-way ANOVA with Tukey’s post hoc test (f) or two-way RM ANOVA with Šidák’s post hoc test (k,l,m,q,r).
