Extended Data Fig. 9: Efficient CD8 memory T-cell formation depends on T-cell-intrinsic ST2 signaling.
From: A type 1 immunity-restricted promoter of the IL−33 receptor gene directs antiviral T-cell responses
a-k, P14, Il1rl1-/- P14 or Il1rl1-ExAB-/- P14 T cells (all CD45.1+) were adoptively transferred into WT mice (CD45.2+). Recipients were infected with LCMV-Cl13 (200 PFU) and analyzed at d30 p.i. (P14: n = 6, P14 Il1rl1-/-: n = 6, P14 Il1rl1-ExAB-/-: n = 5). a, Experimental outline. b-d, Representative FACS plots (b) and quantification (c,d) of memory P14 cells in indicated organs. e-g, Representative FACS plots (e) and quantification (f,g) of splenic effector memory (Tem, CD44+ CD62L−) and central memory (Tcm, CD44+ CD62L+) T-cell subsets. h,i, Representative FACS plots (h) and quantification (i) of P14 cells labeled in vivo by i.v. injection of CD90.2-PE antibody prior to sacrificing mice. j, Representative FACS plots showing CD69 and CD103 expression in i.v.+ and i.v.- P14 cells. k, Counts of i.v.+ and i.v.- P14 cells in spleens, lungs, salivary glands and kidneys of infected animals. Results are presented as mean ± SD with each dot representing one mouse. P was determined using one-way ANOVA with Tukey’s post hoc test (c,d,f,g,k).
