Fig. 3: Usage of distinct promoters allows T-cell subset-specific targeting of ST2 expression.
From: A type 1 immunity-restricted promoter of the IL−33 receptor gene directs antiviral T-cell responses
a, Experimental outline and representative FACS plot showing GFP expression by transduced T cells. b,c, Representative FACS plots (b) and quantification (c) of ST2 surface expression by transduced T cells analyzed after 5 days of culture (n = 6 cultures pooled from two (CTL) or three (Th1, Th2) independent experiments). d, Scheme depicting generation of Il1rl1-ExAB−/− and Il1rl1-ExC−/− mice using CRISPR/Cas9 in murine zygotes. e,f, Representative histograms (e) and quantification (f) of ST2 surface expression by WT, Il1rl1-ExAB−/− or Il1rl1-ExC−/− T cells (n = 4). g, IFN-γ and IL-13 secretion by WT or Il1rl1-ExAB−/− T cells after IL-33 stimulation (n = 3). h–n, WT, Il1rl1−/−, Il1rl1-ExAB−/− and Il1rl1-ExC−/− mice were infected with LCMV-WE (h), and ST2 expression by splenic CTLs (i and j), Th1 cells (k and l) and Treg cells (m and n) was quantified on d7 p.i. (WT: n = 9, Il1rl1−/−: n = 6, Il1rl1-ExAB−/−: n = 8, Il1rl1-ExC−/−: n = 7). In j, l and n, x axis ticks represent the four analyzed genotypes. Group allocation of symbols is depicted in h. Data represent one (g), two (e and f) or three (i–n) independent experiments and are presented as mean ± standard deviation with each dot representing one mouse (j, l and n) or one experiment performed with T cells from individual mice (c and f). P was determined using one-way (j, l and n) or two-way (c and f) ANOVA with Tukey’s post hoc test.
