Extended Data Fig. 3: Generation of Il1rl1-ExAB-/- and Il1rl1-ExC-/- mice.
From: A type 1 immunity-restricted promoter of the IL−33 receptor gene directs antiviral T-cell responses
a, Schematic depiction of the gene-targeting approach for the generation of Il1rl1-ExAB-/- and Il1rl1-ExC-/- mice. b, RNA-seq coverage and splice junction tracks of ST2+ CTLs showing the areas of deletions (gray), T-bet binding sites and ATAC-seq peaks. c, Representative genotyping PCRs to identify heterozygous and homozygous mutant mice. Chromatograms depicting the sequence of joined DNA segments as analyzed by Sanger-sequencing. d-l, Analysis of adaptive and innate immune cells in spleens and lymph nodes (LN) of Il1rl1-ExAB-/+, Il1rl1-ExAB-/- and Il1rl1-ExC-/- mice. d, Representative staining of CD4 and CD8 on splenic T cells. e,f, Frequencies (e) and absolute cell counts (f) of CD8+ T cells, CD4+ T cells and B cells. g, Representative staining of CD62L and CD44 on splenic CD8+ T cells. h, Frequency of effector and central memory CD8+ T cells. i, Frequencies and absolute cell counts of splenic Treg cells. j, Gating strategy for the analysis of innate immune cells. k,l, Frequencies (k) and absolute cell counts (l) of splenic neutrophils, eosinophils, macrophages (MΦ), conventional dendritic cells (cDCs) and natural killer (NK) cells. Data represent two independent experiments and are presented as mean ± SD with each dot representing one mouse (WT, Il1rl1-ExAB-/-, and Il1rl1-ExC-/-: n = 4, Il1rl1-ExAB-/+: n = 5; Treg cell analysis: WT, Il1rl1-ExAB-/-: n = 8 and Il1rl1-ExC-/-: n = 6; NK cell analysis: WT, Il1rl1-ExAB-/- and Il1rl1-ExC-/-: n = 4). P was determined using one-way ANOVA (i,k,l) or two-way AONVA (e,f,h) with Tukey’s post hoc test.
