Extended Data Fig. 5: Extended data on CyTOF analysis in the spleen.

UMAP embedding of the cell clusters identified by FlowSOM clustering (left), heatmap depicting the normalized values of key surface markers across identified clusters (right). (bottom) Frequencies of CD4+ T cell and Ly6Chi monocyte in the spleen and normalized and scaled values of intracellular markers detected by CyTOF. The box plots show median, first and third quartiles and the whiskers show 1.5x interquartile range (IQR) on either side. Statistical analysis was performed by two-tailed Wilcoxon rank-sum test. *, P < 0.05; ***, P < 0.005.