Extended Data Fig. 3: Measuring the longevity of TrPCs.
From: Unraveling the diversity and functions of tissue-resident plasma cells
a, Time stamping data to measure the persistence of Tomato+ ASC from the indicated tissues and populations to identify long-lived PCs. Data are from Fig. 3d and show the mean percentage of ASCs that are of Tomato+ over time. MG: mammary gland, PP: Peyer’s patches, BM: bone marrow, SI: small intestine, VAT: visceral adipose tissue, mesLN: mesenteric lymph nodes, manLN: mandibular LN, SG: salivary glands, GT: genital tract. Each dot is an individual mouse (n = 5). Unpaired t test with Welsh correction (alpha set at 0.05) between the 3-day and 10-month timepoints. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05. Each dot is an individual mouse. Data collected over >3 independent experiments. b, Quantification in TrPCs at 10 months post-tamoxifen treatment, separated by isotype. Each dot is an individual mouse. Each tissue is color-coded. Two-tailed unpaired t test to compare the isotype groups. **** P < 0.0001. c, Flow cytometry contour plots of ASCs from the BM of IgJCreERT2 Rosa26LSL-tdTomato mice 3 days after tamoxifen. Left: co-expression of Blimp1-GFP and the induced dtTomato reporter. Middle-right: gating strategy of IgA, IgG and IgM BMPCs.
