Extended Data Fig. 6: Combined murine spleen and bone marrow PC scRNAseq analysis.
From: Unraveling the diversity and functions of tissue-resident plasma cells
a-g, Two dimensional tSNE plot of single cell transcriptome of murine (CD138+CD98hi) spleen and BMPCs. a, Cell clusters identified by Seurat’ s Louvain algorithm with resolution 0.3. b, Colors identify the tissue of origin (spleen or BM) of each cell. c, Expression of IgJ. d, Percentage of reads mapped to annotated immunoglobulin (Ig) genes out of all reads that were successfully mapped for each cell. Both heavy and light chain genes were included. e, Expression of Ighm (top), Igha (middle) and Ighg (bottom) isotypes. Ighg is the combination of Ighg1, Igh2b, Igh2c, Ighg3 gene expression. f, Expression of maturation-linked genes: Prg2, upregulated in PCs (top), Ssr2 downregulated in PCs (middle), Ccna2 expressed in cycling PBs (bottom). g, Restricted expression of Ccr10 (top) in IgA PCs, Camp (middle) in BMPCs, Chst1 (bottom) in IgM/IgG PCs.
