Extended Data Fig. 4: Identification of glycosylation in the A35Ecto by mass spectrometry.
From: Rational design of a ‘two-in-one’ immunogen DAM drives potent immune response against mpox virus
The glycosylation sites identified by mass spectrometry in the A35Ecto protein (p1-p19) are shown in blue (a). The glycosylation modifications of the smaller band (b) or the larger band (c) in A35Ecto gel electrophoresis were identified by mass spectrometry. The left column shows the detectable glycosylation site, the middle column indicates the mass introduced by each glycosylation modification, and the right column presents the expectation value (the data were deemed reliable with an expectation value of less than 0.01).
