Extended Data Fig. 4: No association between changes in plasmablasts and ICOS+ Tfh cells at Day 10 and responses to both vaccines.
a) Representative plots showing the gating strategy for the characterization by flow cytometry of the different memory CD4+ T cell populations. b) Comparative analysis of the absolute number of the different cell types between PCV13 (n = 16) and PPSV23 (n = 19) cohorts over time. c) Correlation between fold difference in plasmablasts cell numbers (d10 - baseline) and vaccine responsiveness Rank (top panel). Correlation between fold difference in ICOS+ Tfh cell numbers (d10 - baseline) and vaccine responsiveness Rank (bottom panel). The Wilcoxon Rank sum test was used to compare the absolute numbers of different cell types: * (p < 0.05), ** (p < 0.01), ***(p < 0.001), **** (p < 0.0001) (b). The Pearson correlation metric was used to perform correlation analysis (a, b, and c), and the p-value was computed using two-sided t test (c). The ‘n’ represents number of biological replicates.
