Extended Data Fig. 9: The CYTOX Signature stems from CD16+ NK Cells. | Nature Immunology

Extended Data Fig. 9: The CYTOX Signature stems from CD16+ NK Cells.

From: Distinct baseline immune characteristics associated with responses to conjugated and unconjugated pneumococcal polysaccharide vaccines in older adults

Extended Data Fig. 9: The CYTOX Signature stems from CD16+ NK Cells.The alternative text for this image may have been generated using AI.

a) UMAP representation of PBMCs derived from 11 PCV13 donors, consisting of 6 strong responders (SRs) and 5 weak responders (WRs). This visualization encompasses 24 clusters from a total of 52,702 cells, each color-coded by their respective immune cell type. An accompanying feature plot elucidates the distribution of the CYTOX score across these 24 distinct immune cell populations. The CYTOX score is derived from the expression of genes from module associated with PCV13 response (midnightblue module), showing a Pearson correlation coefficient greater than 0.5. b) Subclustering of CD4+ memory cells highlight subsets expressing marker genes for C0 (central memory like cells), C1 (Th1 like), C2 (Th22 like), C3(Th17 like) and C4 (Th2 like). c) A feature plot to show marker gene expression for memory CD4+ subsets. d) A feature plot showing CYTOX score for memory CD4+ T cells. e) Comparison of CYTOX scores for 6 SRs and 5 WRs for each memory CD4+ subset and CD8+ TEMRA CTLs. Boxplots display the median and IQR (25–75%), with whiskers representing the upper- and lower-quartile ±1.5× IQR. The Wilcoxon Rank sum test (two-sided) was employed to assess differences in the CYTOX scores between the PCV13 SRs and WRs across these CD4 memory subsets and CD8+ TEMRA CTLs. The ‘n’ represents number of biological replicates.

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