Extended Data Fig. 3: Differential transcriptional changes in response to PCV13 and PPSV23 at day 10.
a) Heatmap showing differentially expressed genes between Day 10 and baseline, using the normalized gene expression. Differentially expressed genes that are common for both vaccines or show differential expression only in PCV13 or PPSV23 are grouped separately. b) Boxplots of the normalized expression of genes coding for the constant region of immunoglobulin heavy chain structure. c) Heatmap of differential expressed genes coding for the constant region of immunoglobulin heavy chain structure at Day 10 in response to PCV13 and PPSV23. Genes with a FC >1.5-fold difference and a FDR p-value <0.05 are marked with stars. d) Correlation analysis between the fold difference in immunoglobulin genes (gene expression at Day 10 - baseline) and the extent of the response (% of serotypes out of 13) for PCV13. e) Correlation analysis between the fold difference in immunoglobulin genes (gene expression at Day 10 - baseline) and the extent of the response (% of serotypes out of 13) for PPV23. f) Correlation between the plasmablasts activity scores (Day 10 vs. baseline) and vaccine responsiveness Rank. The Pearson correlation metric was used to perform correlation analysis (a, c, and d). The Wilcoxon matched-pairs signed-rank test (two-sided) was used to compare the expression of immunoglobulin genes at baseline and Day 10 for PCV13 and PPSV23 (c). The ‘n’ represents number of biological replicates.
