Extended Data Fig. 2: DAR groups are enriched for distinct TF motifs.

a, Schematic of BALB/c transfer system using VPD-labeled donor cells. Mice were euthanized at days 2.5 and 11.5. b,c Representative flow plots depicting VPD-dilution of listed cell types compared to total transferred B cells in PBS control (unimmunized) at days 2.5 (b) and 11.5 (c). d, PCA plot of genes with expression of >= 0 log2 normalized reads in at least one cell type. e, PCA plot of OCRs with accessibility of >= 1 log2 normalized reads in at least one cell type. f, Ratio of Euclidean distance between DPs and EMPs to Euclidean distance between DNs and EMPs for given log2 FC RNA-seq differential expression thresholds, where Fig. 2c is the reference at abs(log2 FC DP vs DN) >=1. For Euclidean distance, a higher number indicates less similarity; if Euclidean distance ratio is > 1, numerator is less similar than denominator. g, Ratio of Euclidean distance between DNs and LZ GCs to Euclidean distance between DPs and LZ GCs for given log2 FC RNA-seq differential expression thresholds, where Fig. 2c is the reference at abs(log2 FC DP vs DN) >=1. h, Table of top TF motifs enriched in various DAR groups from Fig. 2d compared to background (all called peaks), computed via HOMER. Examples were manually chosen. p = p.value (calculated using cumulative binomial distribution), Motif Score = motif enrichment score above background, Known Motif = motif taken from HOMER database, TF Motif Name = specific TF and/or TF family, along with source if provided, % Coverage in DAR group = % of OCRs in a DAR group that have at least one motif sequence, % Coverage in all OCRs = % of all called OCRs that have at least one motif sequence. i, Heatmap depicting log2 normalized RNA expression of select TFs from Fig. 2f. j, Heatmap depicting all TF motifs with enrichment scores of log2 FC > = 1.4 above background, p. value <= 0.001 for any DAR group. Values shown are motif enrichment scores above background.