Extended Data Fig. 4: Ly6D, PlexinB2, and CD73 can be used to identify GCBC-derived MBCs in WT BALB/c mice. | Nature Immunology

Extended Data Fig. 4: Ly6D, PlexinB2, and CD73 can be used to identify GCBC-derived MBCs in WT BALB/c mice.

From: Memory B cell subsets have divergent developmental origins that are coupled to distinct imprinted epigenetic states

Extended Data Fig. 4

a, Flow cytometry plots depicting markers expressed by YFP+ MBCs in week 6 KP-KLH-immunized GCET-TamCre+/−R26-LSL-YFP+/− mice (from Fig. 3g; tamoxifen given on days 3–13). b-d, NIP+ Memory cells from directly immunized WT BALB/c mice 8 weeks post-NP-CGG immunization. b, Flow cytometry gating strategy to determine GC-derived MBCs (PlexinB2hiLy6DloCD73+) frequencies within CD80/PD-L2 MBC subsets. c, Flow cytometry gating strategy to determine frequencies of CD80/PD-L2 MBC subsets within total GC-derived MBCs (PlexinB2hiLy6DloCD73+). d, top: percent of GC-derived cells within each CD80/PD-L2 MBC subset; bottom: percent of each CD80/PD-L2 subset within total GC-derived MBCs (n = 5). Bars display mean ± s.d.

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