Extended Data Fig. 6: NP-Ficoll does not produce GCBC-derived MBCs. | Nature Immunology

Extended Data Fig. 6: NP-Ficoll does not produce GCBC-derived MBCs.

From: Memory B cell subsets have divergent developmental origins that are coupled to distinct imprinted epigenetic states

Extended Data Fig. 6

a, Number of total MBCs at Day 5 (left) and Day 28 (right) between NP-CGG + PBS (day 5; n = 4, day 28, n = 5), NP-Ficoll + PBS (day 5; n = 6, day 28, n = 4), and NP-Ficoll + GK1.5 (day 5; n = 5, day 28, n = 7) treated mice from Fig. 5. b, Left/middle: Representative flow plots of pre-gated NIP+ B cells from Day 5 post-immunization for NP-KLH + PBS, NP-Ficoll + PBS, NP-KLH + GK1.5, and NP-Ficoll + GK1.5 treated GCET-TamCre+/−R26-LSL-YFP+/− mice. Mice were given one dose of tamoxifen on Day 3; right: Representative flow plots of pre-gated NIP + B cells from Day 28 post-immunization for NP-KLH + PBS, NP-Ficoll + PBS, NP-KLH + GK1.5, and NP-Ficoll + GK1.5 treated GCET-TamCre+/−R26-LSL-YFP+/− mice. Mice were given one dose of tamoxifen every other day from day 3–13. c, Number of YFP+ GCBCs (NIP+CD19+CD138−CD38−GL7+) per spleen at day 5 (n = 3 per group). d, left: Percent YFP+ of DP MBCs (NIP+CD19+CD138−CD38+GL7−), middle: Number of total DP MBCs per spleen, and right: Number of YFP+ DPs per spleen at day 28 (n = 3 per group). Bars display mean ± s.d. P-values were calculated using two-tailed Welch’s t-test (*P < = 0.05, **P < 0.01, ***P < 0.001). Actual p-values are listed in source data.

Source data

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