Extended Data Fig. 10: Validation of CyTOF antibodies.

a, CyTOF analysis of human lymphoid aggregate cultures generated from tonsils depicting CD3⁺ T cells on the top and CD3⁻ B cells on the bottom as indicated, analogous to methods previously described¹⁸. b, CyTOF analysis of PMA/ionomycin- or LPS-stimulated PBMCs, depicting CD3⁺ T cells on the top and CD3⁻ cells on the bottom, similar to prior studies^2,3,4,5. c, Expression of Foxp3 among CD4⁺ T_reg cells and CD4⁺ T_N cells, as assessed by CyTOF. d, Expression of CD30 and Ki67 among CD3⁺ CD45RO⁺CD45RA⁻CD4⁺ T memory (T_M) cells and CD4⁺ T_N cells, as assessed by CyTOF. ****p < 0.0001 (two-sided paired t-test). e, Illustration of pT_FH gate implemented on PBMC samples, and T_FH gate implemented on tonsil samples. Cells were pre-gated on CD4⁺ T_M cells.