Fig. 4: LC improves T cell-based tumor immunotherapy in mice.
From: Lithium carbonate revitalizes tumor-reactive CD8+ T cells by shunting lactic acid into mitochondria
a, Schematic of experimental design for b and c. i.g., qod, intragastric administration, every other day; s.c., subcutaneous; i.v., intravenous. b, B16 tumor growth of C57BL/6J mice gavaged with 75 mg per kg body weight of LC once per 2 d. n = 7 mice. c, Percentage of infiltrating CD8+ T cells in B16 tumor, IFN-γ+ and CD107a+ cells in CD8+ TILs at day 18 after tumor injection in mice. n = 6 mice. d, Schematic of experimental design for e and f. e, Percentage of infiltrating CD45.1+CD8+ T cells in OVA-B16 tumor, IFN-γ+ and CD107a+ cells in CD45.1+CD8+ T cells at day 18 after tumor injection in mice. n = 6 mice. f, OVA-B16 tumor growth of C57BL/6J mice gavaged with LC once per 2 d. n = 8 mice. g, B16 tumor growth of C57BL/6J mice gavaged with LC once per 2 d and treated with a total of 200 μg of CD8α neutralizing antibodies once per 2 d for 10 d. n = 8 mice. h, OT-1 CD8+ T cells transfected with NC-GFP, shMCT1-GFP or shLDHB-GFP plasmids were adoptively transferred into OVA-B16 melanoma-bearing mice gavaged with LC once per 2 d. i, Percentage of OVA-B16-infiltrating CD8+GFP+ T cells in tumor and GFP+IFN-γ+ and GFP+CD107a+ cells in CD8+ TILs at day 18 after tumor injection in mice as in h. n = 8 mice. j, B16 tumor growth of C57BL/6J mice gavaged with LC once per 2 d and treated with a total of 10 μg of αPD-1 antibodies once per 2 d for 10 d. Created with BioRender.com. k, B16 tumor growth (left) and survival (right) of C57BL/6J mice. n = 8 mice. l, Percentage of infiltrating CD8+ T cells in B16 tumor, IFN-γ+ and CD107a+ cells in CD8+ TILs at day 18 after tumor injection in mice as in j. n = 6 mice. Data are presented as the mean ± s.e.m. P values were calculated using unpaired two-tailed Student’s t-test (c), one-way ANOVA for Dunnett’s multiple-comparisons test (l), two-way ANOVA for Sidak’s multiple-comparisons test (b), Tukey’s multiple-comparisons test (f, g, i and k, left), Mann–Whitney test (e) and log-rank test for survival analysis (k right).
