Extended Data Fig. 2: PG16-Db and 3BNC117-Db promote elimination of Env⁺ cells.

a, Binding of PG16-Ig and 3BNC117-Ig to NL4.3-ΔNef-eGFP-infected CD4⁺ T cells. Data reported as the percentage of hIgG Fc⁺ CD4⁺ T cells after preincubation of the cells with 20 μg/mL of PG16-Ig, 3BNC117-Ig, or an equimolar combination of PG16-Ig+3BNC117-Ig (mean, SD) from three replicates. b, Representative flow histograms showing binding of PG16-Ig, 3BNC117-Ig, and PG16-Ig+3BNC117-Ig to GFP⁺ CD4⁺ T cells. All conditions shown are after pre-incubation of the infected CD4⁺ T cells with 20 μg/mL of the corresponding IgG. Data reported as fluorescence intensity. c, CD4⁺ T cells infected with NL4.3- ΔNef-eGFP were co-cultured with autologous NK cells in the presence of H2-Db, PG16-Db, 3BNC117-Db, PG16-Ig, or 3BNC117-Ig at 2000 pM. Data reported as the frequency of viable GFP⁺ cells relative to no scDb/IgG treatment (percent reduction, mean, SD) from three replicates. Significance determined by one-way ANOVA followed by Dunnett’s test for multiple comparisons, ****P < 0.0001. d, CD4⁺ T cells infected with NL4.3 were co-cultured with autologous NK cells in the presence of H2-Db, PG16-Db, 3BNC117-Db, PG16-Ig, or 3BNC117-Ig. Data reported as the frequency of viable p24⁺ cells relative to no scDb/IgG treatment (percent reduction, mean, SD) from three replicates. Significance determined by one-way ANOVA followed by Dunnett’s test for multiple comparisons, ****P < 0.0001.