Extended Data Fig. 6: IL-23 sensing by Treg cells initiates an eTreg cell program in the murine TME.
From: IL-23 stabilizes an effector Treg cell program in the tumor microenvironment
(a-c) Foxp3Cre-YFP/+ (heterozygous) Il23rfl/fl female mice were inoculated i.d. with B16 tumor cells and combined transcriptome (scRNAseq) and protein expression analysis of sorted CD4+T cells was performed on day 13 post-inoculation. (a) Log fold change of DA neighborhoods marked in red, neighborhoods with spatial FDR > 0.1 marked in grey (top). Heatmap depicting average gene expression of most variable markers across neighborhoods (bottom). (b) Dotplot depicting differentially expressed protein markers between the identified Treg cell clusters. (c) Expression density of selected variable features overlayed on UMAP displaying tumor Treg cells.
