Extended Data Fig. 4: The effect of astrocyte-specific HB-EGF abrogation during EAE.
From: The astrocyte-produced growth factor HB-EGF limits autoimmune CNS pathology
a, Significance Analysis of Microarrays (SAM) of FlowSOM clusters from CNS cells obtained from Gfap::Scrmbl and Gfap::Hbegf mice analyzed by high-dimensional flow cytometry. n = 5 per group. b, SAM plot depicting significant alterations in cluster abundance in Gfap::Scrmbland Gfap::Hbegf mice analyzed by high-dimensional flow cytometry. n = 5 per group. c, representative scatter plots and quantification (d) of CD45+CD11b− infiltrating lymphocytes, CD45hiCD11b+ myeloid cells, CD45intCD11b+ microglia, and CD45−CD11b− cells in the CNS of Gfap::Scrmbl and Gfap::Hbegf mice. n = 5 per group. e, gating strategy used for the quantification of cytokines and intranuclear factors by intracellular flow cytometry. f, cytokine expression (% of parent) by major cell populations in the CNS of Gfap::Scrmbl and Gfap::Hbegf mice. n = 4 per group. Data shown as mean ± SD. Two-way ANOVA with Sidak’s multiple comparisons test in (d, f).
