Fig. 1: An integrative transcriptomic atlas of human bone marrow cell states.
From: An immunophenotype-coupled transcriptomic atlas of human hematopoietic progenitors
a, Bone marrow aspirate cell isolation and titration experimental workflow. b, Scheme for the integration of prior reference cell annotation labels (cellHarmony and Azimuth) with unsupervised clustering of 315,792 bone marrow cells using scTriangulate based on RNA. c, Uniform manifold approximation and projection (UMAP) of the source cell isolation enrichment approach for cells from four healthy donors. d, Final scTriangulate clusters annotated by the reference study annotation source. e, scTriangulate stability confidence score from the integration (Shapley confidence score). f, Transcriptome-defined cluster annotations based on the source clusters and marker genes. g–i, Top RNA-defined marker genes in HSPCs (g), presumptive multilineage progenitors (h) and stromal cells (i). Abbreviations: BMCP, basophil/mast cell progenitor; Mk, megakarocyte; Er, erythroid; Myel, myeloid; MultiLin, multi-lineage progenitor; TEM, effector memory T cell; TCM, central memory T cell; MAIT, mucosal-associated invariant T cell; MPP, multipotent progenitors; Eos, eosinophils; MKP, megakaryocyte progenitor; ASDC, AXL+SIGLEC6+ DC; Mono, monocyte; cMOP, common monocyte progenitor; Mac, macrophage; MSC, mesenchymal stem cell; Neu, neutrophil; PC, plasma cell; int, intermediate.
