Extended Data Fig. 3: Previously infected, antibody-deficient hyACE2 knock-in mice are protected against heterologous SARS-CoV-2 re-challenge.
(a) Survival curve of Ab+ (n = 10, blue line) and Ab− hyACE2 upon SARS-CoV-2 infection (n = 10, red line). Mice were exposed to 5 × 105 TCID50 of SARS-CoV-2 B.1.617.2, as described in Fig. 3. (b) Representative flow cytometry plots (left) and frequency (right) of GL-7+ FAS+ B cells in the mLN of indicated mice 4 days post re-challenge (pre-gated on live+/CD4−/CD8−/B220+/CD19+ cells). n as indicated in Fig. 3a. Data are expressed as mean ± SEM. Data are representative of at least 2 independent experiments. *p value < 0.05, **p value < 0.01, ***p value < 0.001; Kruskal-Wallis test followed by uncorrected Dunn’s test, each comparison stands alone (b).
