Extended Data Fig. 3: Anti-tumor efficacy of TRH/TSH/TH alone or combined with anti-PD-1 in B16-F10 tumor-bearing mice.

a, Average tumor growth curves (full day 0 to day 13) of B16-F10 tumor-bearing mice after treatment indicated in Fig. 4e. Data are presented as the means ± s.d. b, Images of tumor tissues at the end of treatments. The cross symbols within rectangles represent no tumor due to tumor volume exceeding ethical limits or actual mouse death. c, Average tumor growth rate of B16-F10 tumor-bearing mice after treatment. Pairwise comparisons by two-sided Mann–Whitney U test, then FDR (BH) for correction of p-values. d, Kaplan-Meier survival plot of B16-F10 tumor-bearing mice after treatment. e, Images of B16-F10 tumor-bearing mice on day 7 and day 13 after the first treatment. f, Histological observation of major organs in B16-F10 mice-bearing mice at the end of treatments by H&E staining. Images are representative of two biologically independent mice. Scale bar: 20 µm. n = 9 for TSH and TH group, n = 8 for other groups (a, c, d).