Extended Data Fig. 5: Characterization of joint inflammation in SHARPIN deficiency.
From: Biallelic human SHARPIN loss of function induces autoinflammation and immunodeficiency
(a) Multiplex ELISA measurement of chemokines in the sterile synovial fluid from P1 before the initiation of anti-TNF treatment, compared with osteoarthritis (OA) control donors (N = 7). The samples were measured in technical triplicate (P1) or duplicate (OA), respectively. Mean values ± s.d are displayed. (b) Quantification of CD45 positive cells in tendons of shoulder joints of control and Sharpin-deficient mice (n = 4 for each group). Data are represented as mean values + SEM. Significance calculated with a two-tailed Mann-Whitney test. (c) Representative hematoxylin and eosin (H&E) staining sections of elbow joints from Sharpin-deficient mice (N = 2) and wild-type littermate controls (N = 2). The arrowhead indicates an inflamed ligament.
