Extended Data Fig. 7: Flt3Cre fate mapping of single HSCs.

a-b, Representative flow cytometry profiles (% of parent gates) of platelets and erythrocytes (a) and leukocyte lineages (b) of a 12 week old steady-state Flt3Cre^tg/+ R26^Tom/+ Vwf-eGFP^tg/+ Gata1-eGFP^tg/+ mouse. P: platelets, E: erythrocytes, M: myeloid cells, B: B lymphoid cells, T: T lymphoid cells. c, CD201, Vwf-eGFP, and Gata1-eGFP expression (mean ± s.e.m. % of parent gate) in BM LSKCD34⁻CD150⁺CD48⁻ HSCs of 9–13 week old steady-state Flt3Cre^tg/+ R26^Tom/+ Gata1-eGFP^tg/+ (n = 2), Flt3Cre^tg/+ R26^Tom/+ Vwf-eGFP^tg/+ (n = 2), and Flt3Cre^tg/+ R26^Tom/+ Vwf-eGFP^tg/+ Gata1-eGFP^tg/+ mice (n = 4). d, e, Representative flow cytometry profiles (% of parent gates) of blood lineages of a mouse reconstituted by a single Vwf-tdTomato⁻ Multi-HSC (d, n = 14) or a single Vwf-tdTomato⁺ P-restricted HSC (e, n = 14) sorted from a Flt3Cre^tg/+ R26^Tom/+ Vwf-eGFP^tg/+ Gata1-eGFP^tg/+ donor. P: platelets, E: erythrocytes, M: myeloid cells, B: B lymphoid cells, T: T lymphoid cells. f, Cell-surface FLT3 and tdTomato (Tom) labelling (mean ± s.e.m. % of parent gate) in BM LSK cells of mice reconstituted by single Vwf-eGFP⁻ Multi-HSCs (n = 14) or Vwf-eGFP⁺ P-restricted HSCs (n = 14) sorted from Flt3Cre^tg/+ R26^Tom/+ Vwf-eGFP^tg/+ Gata1-eGFP^tg/+ donors. Note that all LSKFLT3⁺ cells are Tom⁺ whereas Vwf-eGFP⁺ P-restricted HSCs do not generate LSKFLT3⁺ or LSKTom⁺ cells.

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