Fig. 7: Fate mapping of alternative platelet replenishment pathways.

a, Flt3Cre-tdTomato labeling (mean ± s.e.m.) of blood lineages in steady-state Flt3Cre^tg/+ R26^Tom/+ Vwf-eGFP^tg/+ Gata1-eGFP^tg/+ mice (n = 7; 8–13 weeks old): platelets (CD150⁺CD41⁺TER119⁻), erythrocytes (TER119⁺CD150⁻CD41⁻), myeloid (granulocyte) cells (CD11b⁺GR1⁺CD41⁻NK1.1⁻CD19⁻CD4/CD8a⁻), B lymphocytes (CD19⁺CD41⁻NK1.1⁻CD4/CD8a⁻CD11b⁻GR1⁻) and T lymphocytes (CD4/CD8a⁺CD41⁻NK1.1⁻CD11b⁻GR1⁻CD19⁻). Dots represent individual mice. Tom, tdTomato. b, Representative profiles of Flt3Cre-tdTomato labeling in platelets (left) and mean ± s.e.m. labeling of all lineages (right) replenished by a single CD45.2 Vwf-eGFP⁻ multi-HSC (top; n = 14) and Vwf-eGFP⁺ platelet-restricted P-HSC (bottom; n = 14) 18–21 weeks after transplantation.

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