Extended Data Fig. 4: Flow cytometry demonstrates that clock disruption alters immune proportions.
From: Circadian control of tumor immunosuppression affects efficacy of immune checkpoint blockade
(a) Bar graph of immune cells as percent of live cells from the intestine of WT, Bmal1−/−, Apc+/−, and Apc+/−;Bmal1−/− mice sacrificed at ZT 4 and analyzed by flow cytometry (n = 7 mice/genotype). (b) Bar graph of immune cell counts for neutrophils, monocytes, and macrophages from WT, Bmal1−/−, Apc+/−, and Apc+/−;Bmal1−/− mice sacrificed at ZT 4 and analyzed by flow cytometry (n = 7 mice/genotype). (c) Bar graph of immune cell counts for CD8+ T cells and CD4+ T cells from WT, Bmal1−/−, Apc+/−, and Apc+/−;Bmal1−/− mice sacrificed at ZT 4 and analyzed by flow cytometry (n = 7 mice/genotype). (d) Bar graph of immune cells as percent of live for neutrophils, monocytes, and macrophages from WT, Bmal1−/−, Apc+/−, and Apc+/−;Bmal1−/− sacrificed at ZT 4 and analyzed by flow cytometry (n = 7 mice/genotype). (e) Bar graph of immune cells as percent of live for CD4+ T cells and CD8+ T cells from WT, Bmal1−/−, Apc+/−, and Apc+/−;Bmal1−/− mice sacrificed at ZT 4 and analyzed by flow cytometry (n = 7 mice/genotype).
