Extended Data Fig. 1: Circadian clock disruption promotes intestinal tumorigenesis.
From: Circadian control of tumor immunosuppression affects efficacy of immune checkpoint blockade
(a) Graphic of mouse genotypes. (b) Gene targeting strategy for generation of intestine-specific knockout of Bmal1 and Apc. (c) Small intestinal polyp count from WT, Bmal1−/−, Apc+/−, and Apc+/−;Bmal1−/− mice (n = 15 mice/genotype). (d) Spleen weight from WT, Bmal1−/−, Apc+/−, and Apc+/−;Bmal1−/− mice (n = 15 mice/genotype). (e) Violin plots of RNA features, count, and percent of mitochondrial RNA for each immune cell cluster prior to filtering mitochondrial RNA < 10% and nFeature < 3000. (f) Heatmap of top three genes expressed by each immune cell cluster. Data represent the mean ± SEM and statistical significance was determined by one-way ANOVA with Tukey’s multiple comparison test for C and D. Asterisks represent p-values from multiple comparisons, with **** indicating a p-value of < 0.0001.
