Extended Data Fig. 8: Mouse colonic CD8+ T function was dependent of CD4+ T cells.
From: Stem-like T cells are associated with the pathogenesis of ulcerative colitis in humans
a, Flow-cytometric plots show sequential gating strategy used for isolating TCRβ+CD8β+CD4− T cells from spleen. b, Histology scores for the degree of colon tissue inflammation (n = 5 for CD4+ + CD8+ T cell transfer; n = 6 for CD8+ T cell transfer). c, Representative flow cytometry contour plots and frequency of colon LP CD8+ T cells that expressed the indicated cytokines following ex vivo stimulation with PMA-Ionomycin for 4 hours (n = 5 for CD4+ + CD8+ T cell transfer; n = 6 for CD8+ T cell transfer). Bar graphs in (b,c) depict mean ± SEM, each symbol represents data from an individual mouse; all data from are representative of one of two independent experiments with similar results except that histology in (b) was performed only once. Statistical significance for the comparisons was computed using one-way ANOVA (b), Student’s unpaired two-tailed t test (c).
