Fig. 4: Cellular composition of pan-cancer cell atlas and subset distribution of TiNK cells.
a, Heatmap depicting changes in immune subset proportion in tumor samples compared with healthy tissue samples at the pan-cancer level and within individual tumor types. b,c, Proportions of major immune subsets within healthy tissue (b) and tumor samples (c). d,e, Predicted subset annotations of CellTypist-identified NK cells in healthy tissue (d) and tumor samples (e) compared with annotated PB-NK cells. f,g, Frequency of CD56bright NK cells (f) and relative frequency of subsets (g) identified by flow cytometry in a cohort of healthy blood donors (n = 19) and central tumor samples from patients with NSCLC (n = 25), from 23 independent experiments. Data were analyzed using two-sample Student’s t-test with Bonferroni’s correction (a) and a two-tailed Mann–Whitney U-test (f): *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. The bar graph in f represents the mean ± s.d, with the actual P value indicated (P < 0.0001).
