Fig. 8: Expression of IFNγ solely by hematopoietic cells is sufficient to induce CD8+ T cell tolerance.
WT and Ifng–/– mice on a C57BL/6J background were lethally irradiated and reconstituted with syngeneic WT or Ifng–/– BM cells depleted of T and NK cells. Eleven weeks after reconstitution, the resulting four groups of BM chimeras were immunized with IFNγ69–78 and OVA257–264 peptides using anti-CD40 and poly(I:C) as adjuvant (see also Extended Data Fig. 8c). For quantification of antigen-specific CD8+ T cell responses, splenocytes were isolated 6 days after immunization and stimulated with IFNγ69–78 peptide for 6 h (Methods). Production of TNF by CD8+ T cells was analyzed by flow cytometry (n = 5 for WT → Ifng–/– and n = 4 for the other groups). Data are presented as mean ± s.d. (**P < 0.01) and were analyzed by one-way ANOVA with a Holm–Sidak multiple comparisons test. Data are representative of two independent experiments.
