Extended Data Fig. 4: FACS-sorted HSCs are highly enriched for HSC-specific genes.
From: A stromal inflammasome Ras safeguard against Myc-driven lymphomagenesis
a, Flow cytometry contour plots showing gating on lineage−Sca-1+c−-Kit+ (LSK) stem cells prior to FACS-sorting of cells for RNA-sequencing. LSK cells were isolated from total bone marrow cells extracted from femurs and tibias of indicated premalignant 6-week-old mice and stained for specific LSK cells isolation with an antibody mixture containing the Lineage marker cocktail (clones: 145-2C11, RB6-8C5, M1/70, RA3-6B2, Ter-119), anti-c-Kit (clone 2B8) and anti-Sca-1 (clone D7) antibodies. b,c, Histogram of mean normalized counts of sequenced genes from sorted LSK cells showing high enrichment of genes restricted to HSC as reported by the laboratories of Lemischka and Dick29,30, compared to relatively lower enrichment of HSC genes reported by Rossi laboratory31 (b), and highest enrichment compared to signature HSC genes represented in the Hematopoietic Stem Cell Differentiation gene ontology biological process (b,c). Positioning of the average total counts of genes in our sorted LSK HSC cells is indicated as ‘Total’.
