Extended Data Fig. 7: Frequency of Major Cell Types Across Vaccination Groups.
From: Variants and vaccines impact nasal immunity over three waves of SARS-CoV-2
a) Frequency of B cells, dendritic cells, macrophages, and T cells as a percentage of all cells in the sample in control participants. Control (‘Ctrl’) unvaccinated (‘Unvax’) (n = 13) samples were collected during the Ancestral wave, and control vaccinated (‘Vax’) (n = 13) samples were collected during the Delta and Omicron waves. b) Frequency of epithelial cell types (basal cells, ciliated cells, deuterosomal cells, goblet cells, ionocytes, MThi cells, SARS-CoV-2hi cells (Ctrl Vax vs Ctrl Unvax: p = 0.041), secretory cells, and squamous cells) as a percentage of all cells in the sample in unvaccinated (‘Unvax’) controls (n = 13), vaccinated (‘Vax’) controls (n = 13), unvaccinated Delta (n = 19), vaccinated Delta (n = 14), unvaccinated Omicron (n = 10), and vaccinated Omicron (n = 11) cases. c) Spearman correlation (two-tailed test) between time from most recent SARS-CoV-2 vaccine dose and frequency of immune cell types (B cells, dendritic cells, macrophages, and T cells) in all vaccinated Delta (n = 14) and Omicron (n = 11) cases. Error bands represent 95% confidence interval. a,b Box plots represent median (center line), upper and lower quartiles (box limits); and 1.5x the inter-quartile range (whiskers). Statistical tests represent two-sided Kruskal-Wallis test with Benjamini-Hochberg correction for multiple comparisons. *Dunn’s post hoc test, p < 0.05.
