Fig. 5: The response of NLRP3 variants to MCC950.

a,b, Biplot for ASC₅₀ of NLRP3 variants (INFEVERS database) incubated with or without MCC950 (10 μM) for 4 h (a) or 12 h (b). c–f, ASC speck percentage in the cell population with an increasing amount of NLRP3 expression for WT or T428P or T438A NLRP3 variant with or without MCC950 (10 μM) treatment for 4 h (c,d) or 12 h (e,f). g, Structural annotation of variants that can disrupt the MCC950-binding pocket in the inactive NLRP3 structure (PDB: 7PZC). h, Prediction of ligand affinity between inactive NLRP3 (PDB: 7PZC) and MCC950 (PDB: 8GI) using mCSM-lig. Variants located in the PYD are colored in orange. Variants located in the NACHT domain are colored in blue. The same control data are used when results were generated from the same experiment (c–f). Data were pooled from 3–12 independent repeats (a, n value for each variant is listed in Supplementary Table 1) or 3 independent repeats (c–f), each dot represents the mean value of ASC₅₀ for each NLRP3 variant (a,b) or mean and s.e.m. (c–f).